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. 2007 May 9;14(7):830–838. doi: 10.1128/CVI.00039-07

FIG. 1.

FIG. 1.

Immunoblots demonstrating IgG binding to OMV antigens from the two vaccine strains of pre- and postvaccination sera from one volunteer immunized with three doses of MeNZB. The nitrocellulose strips were incubated without and with 0.02% Tween 20 in the reagents. Strips 1, 3, 5, and 8 were incubated with prevaccination serum; and strips 2, 4, 6, 7, 9, and 10 were incubated with postvaccination serum from volunteer 1043. OMVs from homologous vaccine strain NZ98/254 were antigens for strips 1, 2, 5, 6, and 7; and the heterologous 44/76-SL OMVs were antigens for strips 3, 4, 8, 9, and 10. The positions of the major OMV antigens are shown on both sides of the panel; X designates IgG binding to an unknown antigen visible only in the presence of Tween 20. Specific L1 LPS bands are seen in strips 2 and 6. Strips 7 and 10 were also incubated with 0.15% Empigen BB reagent, which abolished IgG binding to LPS but increased that to PorA, PorB, and/or OpcA. The Adobe Photoshop 8.0 CS program was used to prepare the figure.