TABLE 2.
Comparison between B. garinii and B. burgdorferi IR6 test sensitivitiesa
| Peptide | Sequence | No. positive/total (%)b |
|---|---|---|
| IP90-25 | (C)MKKDDQIAAAMVLRGMAKDGQFALK | 23/55 (41.8) |
| B31-25 | (C)MKKDDQIAAAIALRGMAKDGKFAVK | 31/55 (56.4) |
a
The 25-residue segments correspond to the IR6 immunodominant regions of VlsE in the respective B. burgdorferi sensu lato strains: IP90-25 is B. garinii and B31-25 is B. burgdorferi sensu stricto. (C) is the cysteine appended for purposes of biothinylation. Bold residues in the B. burgdorferi B31 sequence differ from the B. garinii IP90 sequence. The Lyme disease panel used contained 55 acute-phase sera characterized by EM in the first month of infection.
b
The OR for IP90-25 versus B31-25 was 1.787.