FIG. 4.

Western blot analysis for P97c-specific antibody responses. The recombinant P97c protein was submitted to 12% SDS-PAGE and transferred onto a nitrocellulose membrane. Individual strips were incubated with serum (diluted 1:200). Lane 1, preimmune serum; lane 2, serum from rAdGFP-immunized mice; a and b, sera collected at days 14, 30, and 60 postimmunization from mice immunized with rAdP97c via the i.n. or i.m. route, respectively. These sera reacted with the expected protein corresponding to P97c (61 kDa) and also with the product of 26 kDa.