TABLE 1.
Oligonucleotide primer sequences
| Designationa | Nucleotide sequence (5′ to 3′)b | Descriptionc |
|---|---|---|
| P97c546EcoRI(F) | TGATGAATTCGGGGCCTTTAAATCCGTGCTTAATTCCTGGACAGGAAAAATTCAGC | Cloning and SDM |
| P97c546(R) | GTCTAATTTTGCCCAAGGAGCAAAATT | SDM |
| P97c766(F) | AATAATTTTGCTCCTTGGGCAAAATTAGAC | SDM |
| P97c766(R) | CAACCTCTGTTTTCCAATTTTTACCTTG | SDM |
| P97c1054(F) | CAAGGTAAAAATTGGAAAACAGAGGTTG | SDM |
| P97c1054(R) | TTGTCGACTTATTTAGATTCTGGTTCCTC | Cloning and SDM |
| pAd97cBglII(F) | GGAAGATCTGCCACCATGAATTCGGGGCCTTTAAATCC | Cloning |
| pAd97cBglII(R) | GGAAGATCTTTATTTAGATTCTGGTTCCTC | Cloning |
a
The numbers indicate the position of the nucleotide A (in TGA codons) replaced by the nucleotide G during site-directed mutagenesis. F, forward primer; R, reverse primer.
b
The underlined sequences represent EcoRI, SalI, or BglII restrictions sites. GCCACC, Kozak sequence.
c
SDM, site-directed mutagenesis.