TABLE 3.
IFN-γ ELISPOT responses to individual peptides by splenic lymphocytes from BALB/c mice immunized with VRP expressing CMV pp65
| Peptide | Sequence | Responsea |
|---|---|---|
| 3 | PEMISVLGPISGHVL | 1,521 |
| 4 | SVLGPISGHVLKAVF | 1,871 |
| 5 | PISGHVLKAVFSRGD | 778 |
| 10 | HETRLLQTGIHVRVS | 76 |
| 11 | LLQTGIHVRVSQPSL | 69 |
| 27 | SQEPMSIYVYALPLK | 21 |
| 123 | AGILARNLVPMVATV | 44 |
| 124 | ARNLVPMVATVQGQN | 23 |
a
IFN-γ ELISPOT response (number of SFC/106 splenic lymphocytes, average of duplicates) by pooled splenic lymphocytes from groups of six mice immunized at days 0, 21, and 98 and sacrificed at day 189. The mean ± standard deviation response to the other six peptides tested was −3.6 ± 3.1 (range, −8 to 3). The response of cells alone with no peptide was 15.