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. 2007 May 4;6(7):1119–1129. doi: 10.1128/EC.00074-07

FIG. 3.

FIG. 3.

C. albicans Rmt2 catalyzes the formation of δ-MMA as a minor PRMT. (A) C. albicans strains RMT2/rmt2Δ (AMC28; RMT2+) and rmt2Δ/rmt2Δ (AMC30; RMT2−) were labeled in vivo with [3H]AdoMet as described in Materials and Methods. Proteins in cell extracts (50 μg) were analyzed by SDS gel electrophoresis as described in the legend to Fig. 2. The dried gel was exposed to Kodak BioMax MS film at −80°C for 10 days. (B) Amino acid analysis of C. albicans proteins from lysed RMT2/rmt2Δ and rmt2Δ/rmt2Δ cells labeled in vivo with [3H]AdoMet as described in the legend Fig. 2. The upper panel shows the labeling in cells expressing Rmt2 (AMC28; RMT2+), and the lower panel shows the labeling in rmt2Δ/rmt2Δ cells (AMC30; RMT2−). The arrow indicates the peak that decreases in the absence of Rmt2. The radioactivity in the off-scale fractions for the upper panel is 2,062 for fraction 73, 2,759 for fraction 74, and 1,079 for fraction 75; for the lower panel it is 1,973 for fraction 73 and 1,411 for fraction 74. The radioactive derivatives are expected to elute slightly earlier than the standards, as described in the Fig. 2 legend.