TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide | Sequence (5′→3′)a | Site/mutationb |
|---|---|---|
| AM79 | cccatgcatGCGCATCCGGTAGTCGGTGGAGC | NsiI+ |
| AM80 | ccccatATGTCTGAATCAGCTACTGATAAATCAC | NdeI+ |
| AM81 | cccatgcatGCGTAAAAAGTAAGTGTATCCACCC | NsiI+ |
| AM82 | GGCcatATGGAAGTGTCCTGTGGCCAGGCGG | NdeI+ |
| AM113 | cccactagtCCCTTCATTCCGTAATTTCTGTG | SpeI+ |
| AM114 | cccctcgagCAGCTCCAGGGAGGGCTTCG | XhoI+ |
| AM117 | GGTGCATCAGGTGCATCATCAATAACGAC | |
| AM118 | GTGATACCATAACCAGCTCAGCCAGCTC | |
| AM124 | CCCAAACAAGATGTCTGAATCAGC | |
| AM125 | GTTCTGGTGATAAAATAGCTCTCC | |
| AM131 | GCTCTTGGTGGTACTGCTAAAAGTGCCG | |
| AM135 | GAGGCGGCTTCGGTGGGcCCAGAGGTGGATTTGG | ApaI+ |
| AM136 | CCAAATCCACCTCTGGgCCCACCGAAGCCGCCTC | ApaI+ |
| AM141 | CAGAGGTTCTGTCATTACTGTTGAAAGAGATGACAATCCTccaccaccaagaggtagaggaggtttcagaggtag | |
| AM142 | GGCTTACCTGGTTGGTGATCTTTCACGTGGAGCATCTCTgggatcgtatgatccacctctgtcgttg | |
| AM143 | ccccatATGGATGGTCCAGTTGAAGTTACTAAACAG | NdeI+ |
| AM144 | cccggaTCCATAGTACATTCCACTTCTATCTTCTCCG | BamHI+ |
| AM145 | CCAATATAGATTATATCCCAAACAAGATGTCTGAATCAGCTACTGATAAATCACAATTGTC gttttcccagtcacgacgtt | |
| AM146 | GGCTTTGGGGAGAAATATAATAGGGATATACCAGTCTACACATTACGAACACCAATACTA GCtgtggaattgtgagcggata | |
| AM147 | CCATATATCAACTCCTTCCCATTTTCCTTCTTATCTTTCCACCCCCACACACCCCTTATCAAT gttttcccagtcacgacgtt | |
| AM148 | CTTCATAAATTATTTGTCTATCAGCTCCTAATCCATTGAAAAATGAGAAAATCCCATGAGG tgtggaattgtgagcggata | |
| AM154 | ACACAAGACATATTCCTGGGCAGC | |
| AM167 | AACAAACCCACTCATCTCATCAAAA | |
| AM170 | CTGAGCTGGCTGAGCTGGTTATGGTAT | |
| AM173 | GGATACGATCGTGATAACTTCAACGACAGAGGTGGATCATACGATCGTGAAAGATCTCC AACCCGTTTTggtggtggttctaaaggtgaagaattatt | |
| AM199 | GGTCGCTTTATTTCCGTACCGAG | |
| AM203 | GACTACACTCCATTTATTAAAACTGCaATGGAAGAACC | NcoI− |
| AM204 | GGTTCTTCCATtGCAGTTTTAATAAATGGAGTGTAGTC | NcoI− |
| AM208 | CACAGGTTGCAGAAATTTGATACCC | |
| AM213 | GGTATAGAGATGCTGGTTGG | |
| AM214 | GTGGAACTGCACACTACTACTAG | |
| AM244 | GGATATGATAGAGGTGGATACGATCGTGATAACTTCAACGACAGAGGTGGATCATACG ATCGTGAAAGAgCTCCAACCCGTTTTggtggtggttctaaaggtgaagaattatt | |
| AM247 | CAAACTGTAATCTTTTTCTTAATTCCCTTTGGAAACACATCCTAAACGTACTTGATTCTTA GCTGATGTAGtctagaaggaccacctttgattg | |
| AM258 | CCAAAATTGGGACAACACC |
a
Lowercase indicates nucleotide differences from endogenous genes. Introduced or deleted restriction sites are underlined.
b
+, introduced restriction site; −, deleted restriction site.