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. 2007 Apr 20;6(7):1219–1227. doi: 10.1128/EC.00062-07

FIG. 6.

FIG. 6.

Curcumin treatment is associated with reduced gene expression and promoter acetylation. (A) Curcumin treatment (5 and 20 μM) of early trophozoites (18 h) for 12 h resulted in the reduced expression of DNA pol δ, GBP130, actin-related, and calmodulin genes. Shown here are gel pictures of semiquantitative RT-PCR for the PF07_0047 gene (20 cycles) and four other genes (25 cycles). Numbers above each panel indicate average 2−ΔΔCT values of the genes compared to that of PF07_0047 gene by real-time PCR with untreated parasites as the reference (42). (B) Reduced acetylation in the upstream regions of genes. Fragments used for real-time PCR analysis are DNA pol δ (c), GBP130 (d), actin-related (c), and calmodulin (b) as shown in Fig. 4. The region of the PF07_0047 gene was selected from a mapping experiment and the primers are shown in Table S1 in the supplemental material.