Skip to main content
. 2007 May 25;6(7):1228–1238. doi: 10.1128/EC.00036-07

FIG. 5.

FIG. 5.

Genomic organization of TcTREZO. Southern (B to D) and dot blot (E) hybridizations of genomic DNA of different T. cruzi strains and trypanosomatids with 32P-labeled TcTREZO. (A) Schematic representation of TcTREZO elements in the contig with GenBank accession no. AAHK01000548. The DraI, PstI, and EcoRI restriction sites are indicated. (B) Southern blot containing genomic DNA of T. cruzi (clone CL Brener) digested to completion with HindIII (H), EcoRI (E), or BamHI (B) or with two restriction enzymes (H/B, HindIII plus BamHI; H/E, HindIII plus EcoRI; B/E, BamHI plus EcoRI). (C) DNA of T. cruzi (clone CL Brener) digested partially (Ep) or to completion (Et) with EcoRI. (D) Southern blot of genomic DNA of different isolates of T. cruzi (clone CL Brener [CLB], strain Y, clone Sylvio [S], and strain G) digested with EcoRI. (E) Demonstration of species specificity of TcTREZO by dot blot hybridization using genomic DNA from Trypanosoma rangeli (Tr), Trypanosoma brucei (Tb), L. (L.) amazonensis (LLa), and T. cruzi (Tc). Samples were hybridized with randomly primed 32P-labeled TcTREZO. After exposure, the membranes were incubated at 100°C for 30 min in 0.1% SDS and rehybridized with the tubulin gene probe.