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Bro1 immunoprecipitates with the ESCRT-I complex component Vps23. Anti-HA immunoprecipitates (IP: α-HA) from lysates of cells expressing Vps23-13Myc (EN156) transformed with the empty control (pYeF1), HA-Bro1 (pEN001), or HA-Bro1ΔPRD (pEN079) plasmid were immunoblotted with anti-HA (WB: α-HA) to detect Bro1 and Bro1ΔPRD or with anti-c-myc antibodies (WB: α-c-myc) to detect Vps23. Lysate samples were collected before immunoprecipitation.
