TABLE 1.
Yeast strains used in this study
| Straina | Genotype | Source or reference |
|---|---|---|
| 23344c | ura3 | Laboratory collection |
| 27092a | bro1Δ ura3 | 65 |
| 27086c | npi3/bro1ΔPRDura3 | 65 |
| ME029 | snf7Δ ura3 | 47 |
| ME027 | vps4Δ ura3 | 47 |
| EN079 | vps4Δ bro1Δ ura3 | This study |
| EN082 | snf7Δ bro1Δ gap1Δ ura3 | This study |
| EN035 | GAL-GFP-BRO1 ura3 | This study |
| EN040 | GAL1-GFP-BRO1ΔPRD ura3 | This study |
| THY.AP4 | lexA-lacZ-TRP1 lexA-HIS3 lexA-ADE2 ura3 leu2 | 49 |
| THY.AP5 | lexA-ADE2 leu2 trp1 his3 | 49 |
| EN155 | DOA4-13Myc | This study |
| EN156 | VPS23-13Myc ura3 | This study |
| OS20-3 | doa4Δ ura3 | This study |
| 36329a | doa4Δ vps4Δ gap1Δ ura3 | This study |
| 27040d | npi2/doa4W782Rura3 | 64 |
| 27061b | ura3 trp1 | Laboratory collection |
a
Except for those in boldface, strains are congenic with Σ1278b.