TABLE 2.
Plasmids used in the mbSUS assay
| Plasmid | Gene insertion | Reference |
|---|---|---|
| pNubWT-2 | NubWt-3HA | 49 |
| pNXgate | NubG-3HA | 49 |
| pMetYCgate | Met25-Cub-PLV | 49 |
| pEN114 | NubG-3HA-BRO1 | This study |
| pEN133 | NubG-3HA-BRO1ΔPRD | This study |
| pCJ167 | NubG-3HA-PRD-BRO1a | This study |
| pEN120 | NubG-3HA-DOA4 | This study |
| pEN129 | NubG-3HA-DOA4W782R | This study |
| pEN118 | NubG-3HA-SNF7 | This study |
| pEN113 | NubG-3HA-RSP5 | This study |
| pEN141 | NubG-3HA-VPS27 | This study |
| pEN117 | NubG-3HA-VPS23 | This study |
| pEN144 | NubG-3HA-VPS36 | This study |
| pEN106 | Met25-BRO1-Cub-PLV | This study |
| pEN165 | Met25-BRO1ΔPRD-Cub-PLV | This study |
| pEN110 | Met25-DOA4-Cub-PLV | This study |
| pEN164 | Met25-DOA4W782R-Cub-PLV | This study |
| pEN108 | Met25-SNF7-Cub-PLV | This study |
| pEN105 | Met25-RSP5-Cub-PLV | This study |
| pEN157 | Met25-VPS27-Cub-PLV | This study |
| pEN107 | Met25-VPS23-Cub-PLV | This study |
| pEN152 | Met25-VPS36-Cub-PLV | This study |
a
Amino acids 702 to 844.