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. 2007 Jun 1;6(8):1486–1496. doi: 10.1128/EC.00120-07

FIG. 1.

FIG. 1.

Effect of 4-MU on C. neoformans binding activity to HBMEC. An in vitro binding assay was performed and analyzed as described in Materials and Methods. A range of concentrations of 4-MU (0, 0.5, 1.0, and 2.0 mM) was used to treat C. neoformans B-4500FO2 (solid bars) or C559 (open bars) cells for 2 h; then cells were washed with PBS three times prior to the in vitro binding assay. Statistical analysis, using linear regression, shows P values of 0.01 and 0.004 in comparison to the 0 mM group for strains B-4500FO2 and C559, respectively.