Skip to main content
. 2007 Jun 1;6(8):1486–1496. doi: 10.1128/EC.00120-07

FIG. 2.

FIG. 2.

Effect of hyaluronidase on C. neoformans binding activity to HBMEC. A titration amount of hyaluronidase (0. 0.5, 1, and 5 U/ml) was used to treat different C. neoformans strains. An in vitro binding assay was then performed. For the purpose of comparison, we assigned the no-treatment control for each individual Cryptococcus strain a binding level of 100%. Strains used were B-3501 (wild type), TYCC33 (cap59), B4476FO5 (CAP59), 602 (cap64), and TYCC38-602 (CAP64). Data, expressed as percentages of the binding activity of the inoculum, are means of triplicates. Error bars, standard deviations. Statistical analysis of covariance using each individual strain as a grouping variance and titration as a continuous variable revealed a significant linear concentration effect (P = 0.001), with no statistical difference between the strains (P > 0.05). Thus, the level of binding of C. neoformans cells to HBMEC decreased after hyaluronidase treatment.