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. 2007 Jun 1;6(8):1486–1496. doi: 10.1128/EC.00120-07

FIG. 8.

FIG. 8.

Staining of hyaluronic acid fibrous structures outside the C. neoformans cell wall. Yeast strain B-4500FO2 (wild-type CPS1) and its isogenic cps1Δ strain (C559) were probed with biotinylated hyaluronic acid-binding protein and a streptavidin-HRP conjugate. Treated samples were subjected to TEM studies to determine their morphological features. (A) Strain B-4500FO2 shows fibrous structures radiating from the cell wall. Bar, 100 nm. (B) Strain C559 shows only a light patch background near the cell wall (no fibrous structure). Bar, 100 nm. (C) Strain B-4500FO2 cells show a high-density fibrous structure between cells. Bar, 1 μm. (D) Higher magnification of boxed area in panel C. (E) In contrast, strain C559 cells do not show any fibrous structure. (F) Higher magnification of boxed area in panel E.