FIG. 2.
Expression of a UASADE5,7-lacZ reporter is compromised in SAGA and SWI/SNF mutants. The wild-type strain (BY4741/BY4742) and isogenic strains with mutations in subunits of SWI/SNF (snf2Δ, snf11Δ, and taf14Δ strains) and SAGA (gcn5Δ, spt3Δ, spt8Δ, and ada3Δ strains) were transformed with plasmid pR133 (44), which carries the UASADE5,7-lacZ reporter. Strains were grown in SC medium containing adenine (open bars) or lacking adenine (filled bars). Extracts were prepared from cell pellets, and β-galactosidase activities were measured in duplicate assays on at least three individual isolates. Error bars indicate standard deviations.