FIG. 4.

Expression of BAS1 is not affected in strains with mutations in SAGA and SWI/SNF. (A) The isogenic wild-type (WT) strain and strains carrying mutations in the gcn5 and spt20 genes of SAGA and the snf2 and snf5 genes of SWI/SNF were grown in SC medium containing adenine and SC medium lacking adenine. Total RNA was purified from harvested cells, blotted onto nylon membranes, and hybridized to radiolabeled probes for BAS1 and ACT1. This experiment was performed twice, and results from a representative Northern analysis are shown. (B) The results were quantified by phosphorimaging analysis, and the BAS1/ACT1 ratio was normalized to the wild-type ACT1 level. Samples were analyzed in duplicate, and the error bars indicate the ranges. Open bars, SC medium containing adenine; filled bars, SC medium lacking adenine.