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. 2007 Jun 15;6(8):1411–1420. doi: 10.1128/EC.00167-07

FIG. 4.

FIG. 4.

Tagged α1-tubulin gene constructions (α1-tubΔ/ARG7). (A) An XbaI-BamHI restriction fragment containing the ARG7 gene was inserted at the BamHI restriction enzyme site upstream of the 5′ end of the tagged α1-tubulin gene. The tagged α1-tubulin genes had various lengths of 5′ sequence upstream of the transcription start site, indicated by negative numbers. (B) Structure of the resident α1-tubulin gene. The 742-nt antisense RNA probe used in RNase protection assays hybridizes to sequences in the 3′ end of both resident and tagged α1-tubulin RNAs, as indicated above the gene in B, and protects fragments 710 nt from the resident α1-tubulin gene and 554 nt from the tagged α1-tubulin gene. Symbols: +++ to ++, relative magnitudes of the tagged-gene and resident-gene inductions; +, basal level of expression; +1, transcription start site; ATG . . . , translation start site; 233bpΔ and open box with Δ, deletion that tags all α1-tubulin gene constructions; (Δ), deletion between bp −156 and −122 of the −276Δ α1-tubΔ/ARG7 construct; hatched boxes, α1-tubulin gene exons; black boxes, α1-tubulin gene introns; gray box, ARG7 gene.