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Autoradiograph of a representative RNase protection assay. Bands show protected fragments of α1-tubulin resident and tagged RNAs and pcf8-13 RNA isolated from pooled cell lines transformed with −176 α1-tubΔ/ARG7 plasmids and tagged RNA isolated from a single −85 α1tubΔ/ARG7 transformed cell line. RNA samples were isolated from nontreated (NT) and Li+-treated (Li+) cells at the indicated times after the addition of LiCl to 50 mM.
