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. 2007 Jun 1;6(8):1330–1338. doi: 10.1128/EC.00069-07

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Copyright © 2007, American Society for Microbiology

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FIG. 1. — Functional analysis of FLAG₃/TAP-tagged Candida EST2. (A) Telomeres from successive streaks of an est2Δ/est2Δ (est2-ΔΔ) strain and an est2Δ/est2Δ EST2-FLAG₃/TAP strain were analyzed by Southern blotting. The streaks from which the DNAs were derived are indicated at the top. Arrowhead indicates the emergence of a long telomere. (B) The expression of FLAG₃/TAP-tagged Candida Est2p was analyzed by IgG-Sepharose pulldown and Western blotting. For comparison, an extract derived from strain BWP17 (untagged) and an extract containing protein A-tagged S. cerevisiae Est2p were assayed in parallel. IP, immunoprecipitation. (C) Extracts prepared from BWP17 and a strain with FLAG₃/TAP-tagged Est2p were subjected to IgG-Sepharose pulldown, and the telomerase activity in the beads was analyzed by direct primer extension assays.