FIG. 3.

Influence of GlcA negative charges (A), a monovalent cation (B), a chelating agent (C), and a divalent cation (D) on the viscosity of isolated GXM. (A) A reduction of around 95% of the content of GlcA in GXM was associated with a 90% decrease in polysaccharide viscosity (P < 0.05). Viscosity decreased in a dose-dependent way when Na⁺ (B) and EDTA (C) were used. Low Ca²⁺ concentrations induced an increase in polysaccharide viscosity, which was reversed with large amounts of the divalent ion (D). Results are means of three different measurements ± standard deviations.