TABLE 1.
SNPs and nucleotide diversity at coding regions in nine G. lamblia isolates
| Gene | Group | Isolate | No. of haplotypes | No. of SNPs | π (104)a | Tajima's Db |
|---|---|---|---|---|---|---|
| Actin | A-1 | WB | 2 | 1 | 1.6 | −1.16 (NS) |
| Gen | 1 | 0 | 0 | NA | ||
| CAT | 2 | 1 | 2 | −1.16 (NS) | ||
| Be-2 | 1 | 0 | 0 | NA | ||
| A-2 | AB | 4 | 5 | 9.6 | −1.78 (NS) | |
| N | 2 | 1 | 1.6 | −1.16 (NS) | ||
| B | GS | 3 | 2 | 3.6 | −1.51 (NS) | |
| CM | 3 | 26 | 44.6 | −2.48*** | ||
| Be-1 | 1 | 0 | 0 | NA | ||
| Beta giardin | A-1 | WB | 1 | 0 | 0 | NA |
| Gen | 1 | 0 | 0 | NA | ||
| CAT | 2 | 1 | 2.2 | −1.13 (NS) | ||
| Be-2 | 1 | 0 | 0 | NA | ||
| A-2 | AB | 1 | 0 | 0 | NA | |
| N | 1 | 0 | 0 | NA | ||
| B | GS | 2 | 1 | 6.5 | 1.43 (NS) | |
| CM | 3 | 46 | 93.7 | −2.27*** | ||
| Be-1 | 2 | 39 | 119.5 | −1.88** | ||
| CPN60 | A-1 | WB | 2 | 1 | 3.1 | −1.14 (NS) |
| Gen | 2 | 1 | 2.7 | −1.16 (NS) | ||
| CAT | 5 | 4 | 9.8 | −1.84* | ||
| Be-2 | 3 | 2 | 4 | −1.51 (NS) | ||
| A-2 | AB | 2 | 1 | 2 | −1.17 (NS) | |
| N | 1 | 0 | 0 | NA | ||
| B | GS | NDc | ND | ND | ND | |
| CM | ND | ND | ND | ND | ||
| Be-1 | 4 | 4 | 7.9 | −1.86* | ||
| [2Fe-2S] ferredoxin | A-1 | WB | 1 | 0 | 0 | NA |
| Gen | 1 | 0 | 0 | NA | ||
| CAT | 1 | 0 | 0 | NA | ||
| Be-2 | 4 | 3 | 12.2 | −1.35 (NS) | ||
| A-2 | AB | 2 | 2 | 5.3 | −1.51 (NS) | |
| N | 1 | 0 | 0 | NA | ||
| B | GS | 9 | 9 | 29.5 | −2.03* | |
| CM | 10 | 17 | 43.1 | −2.42*** | ||
| Be-1 | 3 | 2 | 5 | −1.51 (NS) | ||
| RPL | A-1 | WB | 1 | 0 | 0 | NA |
| Gen | 2 | 1 | 4.2 | −0.56 (NS) | ||
| CAT | 1 | 0 | 0 | NA | ||
| Be-2 | 1 | 0 | 0 | NA | ||
| A-2 | AB | 2 | 1 | 2 | −1.16 (NS) | |
| N | 1 | 0 | 0 | NA | ||
| B | GS | 6 | 58 | 142.7 | −2.39** | |
| CM | 2 | 1 | 2.2 | −1.16 (NS) | ||
| Be-1 | ND | ND | ND | ND | ||
| TPI | A-1 | WB | 1 | 0 | 0 | NA |
| Gen | 2 | 1 | 4.5 | −1.14 (NS) | ||
| CAT | 3 | 2 | 3 | −1.51 (NS) | ||
| Be-2 | 2 | 2 | 3.5 | −1.51 (NS) | ||
| A-2 | AB | 3 | 3 | 10.1 | −0.96 (NS) | |
| N | 1 | 0 | 0 | NA | ||
| B | GS | 2 | 5 | 40.7 | 2.61** | |
| CM | 1 | 0 | 0 | NA | ||
| Be-1 | 3 | 128 | 781.2 | 1.62 (NS) |
a
π was estimated as described in Materials and Methods.
b
Tajima's D was calculated as described in Materials and Methods. NA, not applicable (in instances where D could not be calculated due to the lack of SNPs); NS, not significant. The asterisks indicate significance of Tajima's D test with the following confidence levels: *, P < 0.05; **, P < 0.01; ***, P < 0.001.
c
ND, not determined.