Skip to main content
. 2007 Jun 15;6(8):1363–1372. doi: 10.1128/EC.00165-07

FIG. 5.

FIG. 5.

Cdc37 levels and protein interactions in the dominant CDC37 mutants. (A) Protein extracts of the indicated strains were separated by polyacrylamide gel electrophoresis, transferred onto nitrocellulose, and probed with anti-Cdc37 antibody. cdc37Δ (high-copy-number [HC] CDC37+) (MRY4), cdc37Δ (low-copy-number [LC] CDC37-109) (MRY5), cdc37Δ (low-copy-number CDC37+) (MRY8), cdc37Δ (low-copy-number CDC37-101) (MRY11), and CDC37+ (low copy number) (MRY10) strains were used. (B) Interaction of Ste11 with wild-type and mutant Cdc37. Whole-cell extracts were incubated with Ni-nitrilotriacetic acid to purify His6-tagged Ste11 proteins and separated by polyacrylamide gel electrophoresis before sequentially Western blotting with antisera to Ste11, Hsp90, and Cdc37. Extracts were made from CDC37+ (MRY73), CDC37-106 (MRY75), CDC37+ His6-V5-Ste11ΔN (MRY79), and CDC37-106 His6-V5-Ste11ΔN (MRY81) strains.