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. 2007 Jun 15;6(8):1363–1372. doi: 10.1128/EC.00165-07

FIG. 6.

FIG. 6.

Functional interaction between PKA and the Hsp90-chaperone complex. (A) Synthetic lethal interaction between Hsp90 subunit gene STI1 and TPK2. The indicated strains were streaked onto minimal medium agar lacking uracil (−URA) or minimal medium agar containing uracil and 5-fluoroorotic acid (FOA) to select against plasmids expressing URA3 and incubated at 23°C. tpk2-62 sti1Δ (pCDC37-101) (MRY47) and tpk2-62 STI1 (pCDC37-101) [SGY562(pGS224)] strains were used. (B) Sti1 is essential for tpk2(Ts) suppression by CDC37-101. Strains were streaked onto minimal medium agar lacking uracil and incubated at 23°C and 34°C for 2 days. TPK2 sti1Δ CDC37+ (ACY77-3), tpk2-62 sti1Δ CDC37-101 [SGY562(pGS224)], tpk2-62 STI1 CDC37+ [SGY562(pRS316)], and tpk2-62 sti1Δ CDC37-101 (MRY47) strains were used.