FIG. 2.

Schematic of the amino acid sequence of the M. catarrhalis ETSU-9 UspA2H protein with the positions of the 5-aa insertions introduced by transposon-mediated mutagenesis indicated by inverted triangles. The numbers flanking each triangle refer to the UspA2H amino acids that flank the insertion site, using the complete UspA2H protein, including the leader peptide, for the numbering scheme. Three insertions (i.e., 81, 92, and 103) resulted in subsequent in-frame deletions of uspA2H DNA; the extent of each deletion is indicated. Mutant ETSU-9.mut20 contained a 15-nt insertion in the DNA immediately 3′ of the translational termination codon of the uspA2H gene.