FIG. 3.

Biofilm formation and Chang cell attachment ability of mutants with 5-aa insertions in the UspA2H protein. (A) Biofilm formation by these uspA2H insertion mutants as measured in the crystal violet-based assay. (B) Attachment to Chang cells by the uspA2H mutants that also possessed a uspA1 mutation. All insertion mutants in panel B are streptomycin resistant and do not express UspA1. ETSU-9.mut20.1 is both streptomycin resistant and a uspA1 mutant that possesses a 15-nt insertion immediately downstream of the uspA2H ORF (see Fig. 2); it is included as a control for the 15-nt insertion. These data represent the means and standard errors from three independent experiments.