FIG. 5.

Biofilm formation by a recombinant E. coli strain expressing the M. catarrhalis UspA2H protein. (A) E. coli EPI300 cells containing a cloned wild-type ETSU-9 uspA2H gene (in pMPE9U2H) or a kan gene (in pCC1kan) were used in the crystal violet-based biofilm assay. These data represent the means and standard errors from three independent experiments. (B) Whole-cell lysates of the same recombinant strains were probed in Western blot analysis with MAb 17C7 to detect UspA2H protein expression. A molecular mass position marker (in kDa) is on the left.