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. 2007 Jun 11;75(9):4357–4363. doi: 10.1128/IAI.01660-06

FIG. 1.

FIG. 1.

Characterization of gastric infiltrating lymphocytes isolated from gastric biopsy specimens from H. pylori-infected subjects. (A) Suspensions of mononuclear cells were isolated from gastric biopsy specimens as described in Materials and Methods. Immediately after isolation, cells were stained for CD3, CD4, CD8, CD69, CD45 RA, and CD45RO and analyzed by flow cytometry. The numbers indicate the percentages of cells detected in each quadrant in the flow cytometric scatter plots. The data are representative of the phenotype observed in gastric infiltrating lymphocytes isolated from 36 infected subjects. (B) Percentages of CD4, CD8, CD45RO, CD45RA, and CD69 expression in CD3+ gastric T cells from the 36 H. pylori-infected subjects mentioned above and 14 uninfected subjects. The data are the means and standard errors of the means. Statistical analysis by the Mann-Whitney rank sum test revealed significant differences in the expression of CD4 and CD8 in gastric T cells between infected and uninfected samples (asterisk, P < 0.01).