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. 2007 Jun 25;75(9):4326–4333. doi: 10.1128/IAI.00455-07

FIG. 3.

FIG. 3.

PAR-1 and PAR-2 gene expression in response to P. gingivalis supernatant in the presence and absence of siRNA. (A) PAR-1 gene expression was evaluated by real-time PCR in response to cell-free supernatant from P. gingivalis and TLCK-treated supernatant in untransfected cells and cells transfected with siRNA targeting PAR-1. Expression of PAR-1 mRNA was significantly down-regulated in response to P. gingivalis supernatant compared to that in unstimulated cells (P = 0.05). PAR-1 mRNA was significantly lower after stimulation with the supernatant as well as with TLCK-pretreated supernatant in all siRNA-transfected cells (P < 0.05) compared to untransfected stimulated and unstimulated gingival epithelial cells. Both controls, bacteria medium and the lipid carrier (HiPerFect), showed no effect on the gene expression of PAR-1. (B) The mRNA expression of PAR-2 was significantly up-regulated in response to cell-free supernatant from P. gingivalis compared to that in unstimulated cells (P = 0.004). PAR-2 mRNA was significantly lower after stimulation with the supernatant as well as with TLCK-pretreated supernatant in all siRNA-transfected cells (P < 0.05) compared to untransfected stimulated and unstimulated gingival epithelial cells. Both controls, bacterial medium and the lipid carrier (HiPerFect), showed no effect on the gene expression of PAR-2. The gene expression study was performed with triplicate samples derived from two to five different donors. *, significant difference (P ≤ 0.05). Error bars indicate standard deviations.