Skip to main content
. 2007 Jun 25;75(9):4326–4333. doi: 10.1128/IAI.00455-07

FIG. 4.

FIG. 4.

hBD-2 mRNA expression in response to P. gingivalis supernatant and effects of siRNA for PAR-1 and PAR-2 in gingival epithelial cells. (A) The mRNA expression of hBD-2 was significantly up-regulated in response to the supernatant (P = 0.02). Stimulation of gingival epithelial cells with TLCK-pretreated supernatant and the control with complete bacterial media showed no significant change in hBD-2 mRNA expression compared to that in the unstimulated control. (B) The gene expression of hDB-2 was up-regulated in PAR-1 gene knockdown cells in response to the supernatant compared to that in untransfected unstimulated cells. No difference between nontransfected and transfected cells was demonstrated in response to P. gingivalis supernatant (P = 0.325). This effect was completely abolished by TLCK pretreatment (P = 0.025). In contrast, in PAR-2 gene knockdown cells, the mRNA expression of hBD-2 showed significantly lower levels in response to the supernatant compared to that in untransfected stimulated cells (P = 0.024). The gene expression study was performed with triplicate samples derived from two to five different donors. *, significant difference (P ≤ 0.05). Error bars indicate standard deviations.