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. 2007 Jun 25;75(9):4326–4333. doi: 10.1128/IAI.00455-07

FIG. 5.

FIG. 5.

CCL20 mRNA expression in response to P. gingivalis supernatant and effects of siRNA for PAR-1 and PAR-2 in gingival epithelial cells. (A) The mRNA expression of CCL20 was significantly up-regulated in response to the supernatant (P = 0.05). Stimulation of gingival epithelial cells with TLCK-pretreated supernatant and the control with complete bacterial media showed significantly lower CCL20 mRNA expression levels compared to stimulation with cell-free supernatant from P. gingivalis (P = 0.05 and P = 0.048, respectively). (B) The gene expression of CCL20 was significantly up-regulated in PAR-1 gene knockdown cells in response to the supernatant compared to that in untransfected unstimulated cells (P = 0.013). No difference between nontransfected and transfected cells was demonstrated in response to P. gingivalis supernatant (P = 0.976). This effect was completely abolished by TLCK-pretreatment (P = 0.006). In contrast, in PAR-2 gene knockdown cells, the mRNA expression of CCL20 showed significantly lower levels in response to the supernatant compared to that in untransfected stimulated cells (P = 0.05). The gene expression study was performed with triplicate samples derived from two to five different donors. *, significant difference (P ≤ 0.05). Error bars indicate standard deviations.