TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Relevant genotype or phenotype	Source or reference
E. coli strains
DH5	F−recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1	Vicky Shingler
S17-1λpir	recA thi pro hsdRM+ Smr <RP4-2-Tc-Mu-Ku-Tn7> Tpr	75
BL21(DE3) plysS	F−dcm lon ompT hsdS (rB− mB−) gal λ(DE3) [pLysS Cmr]	Promega
Y. pseudotuberculosisa strains
YPIII/pIB102	yadA::Tn5 Kmr (parent)	4
YPIII07/pIB102	cpxA in-frame deletion of codons 41-449 Kmr	6
YPIII07/pIB75	yscU in-frame deletion of codons 25-329 Kmr	This study
YPIII08/pIB102	cpxR in-frame deletion of codons 11-193 Kmr	6
SF104/pYH7	ΔyadA (in frame) inv::kan Kmr	30
Plasmids
pLS13	669-bp PCR fragment of the allele encoding YscUΔ25-329 on pDM4; Cmr	43
pCR4-TOPO	TA cloning vector; Kmr Cbr	Invitrogen
pMMB208	Expression vector; Cmr	50
pKEC021	∼700-bp XbaI/KpnI PCR fragment of cpxR in pMMB208; Cmr	6
pJF015	∼720-bp XbaI/KpnI PCR fragment of cpxR encoding D51A mutation in pMMB208; Cmr	6
pET22b(+)	Expression vector; Cbr	Novagen
pMF581	1,381-bp NdeI/EagI PCR fragment of cpxA in pET22b(+); Cbr	6
pKEC017	∼700-bp NdeI/XhoI PCR fragment of cpxR in pET22b(+) that creates a C-terminal His6 fusion; Cbr	This study
pGN37	pBAD18 rovA+; Cbr	31
pIRR1	∼4.4-kb BamHI fragment of inv in pACYC184; Cmr	70

^aTo the best of our knowledge, the Y. pseudotuberculosis strains are all isogenic according to information from literature resources (30, 36, 70).