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. 2007 Jul 16;75(9):4490–4497. doi: 10.1128/IAI.00288-07

FIG. 4.

FIG. 4.

Site-directed mutagenesis of sodC. (A) PCR product of sodC gene. Lanes: 1, sodC::EZ-Tn5; 2, wild-type sodC. The sizes on the left are in bp. (B) Western blot analysis of Cu,Zn SOD in A. actinomycetemcomitans. Lanes: 1, strain DF2200; 2, sodC mutant strain NB9; 3, Cu,Zn SOD (2.5 μg). (C) Survival of A. actinomycetemcomitans after exposure to ROS. The values represent the means of the results of duplicate experiments. The data are expressed as percentages of the time zero values. Black bars, 0 min; gray bars, 15 min.