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Exposure of A. actinomycetemcomitans leukotoxin to ROS and RNS. (A) LtxA (4 μg) treatment at 37°C. Lanes: 1, LtxA alone after 20 min; 2, LtxA alone after 40 min; 3, LtxA with ROS and RNS after 20 min; 4, LtxA with ROS and RNS after 40 min. (B) LtxA (2 μg) treatment for 40 min at 37°C. Lanes: 1, Purified LtxA; 2, LtxA with ROS, RNS, and Cu,Zn SOD (0.3 μg); 3, LtxA with ROS, RNS, and Cu,Zn SOD (0.6 μg); 4, LtxA with ROS, RNS, and Cu,Zn SOD (1.2 μg). Molecular mass standards are in kDa.
