TABLE 1.
Purification step | Total protein (mg) | Sp act (% inhibition rate per μg of protein)a | Fold purification |
---|---|---|---|
Sonicated cell extract | 15.6 | 0.3 | 1.0 |
Heated soluble extract | 3.7 | 12.3 | 4.2 |
Talon column eluate (desalted) | 0.3 | 84.1 | 62.4 |
Inhibition rate of formazan dye reduction with a superoxide anion.