FIG. 2.
Expression of yabJ-spoVG in S. aureus. (A) Schematic representation of the yabJ-spoVG region of S. aureus COL. ORFs, promoters, terminators, and the probes used are indicated. nt, nucleotide. (B) Northern blot analyses of the yabJ and spoVG transcriptions in Newman and SM2 (ΔspoVG) during growth in LB. (C) Effect of σB on yabJ and spoVG expression. Northern blot analyses of the yabJ and spoVG transcriptions in strains GP268, COL, and Newman and their isogenic ΔrsbUVW-sigB mutants after 8 h of growth. Relevant transcript sizes are indicated. Ethidium bromide-stained 16S rRNA patterns are shown as an indication of RNA loading.