FIG. 7.
Capsule production in Newman and its derivatives grown for 8 h in LB at 37°C. (A) CP-5 expression determined by indirect immunofluorescence of strain Newman and its derivatives SM2 (ΔspoVG), SM99 (arlR), and IK184 (ΔrsbUVW-sigB). (B) trans-Complementation of ΔspoVG mutant SM2. CP-5 expression was determined by indirect immunofluorescence of SM2, and SM2 transformed with either pSTM05 (PyabJ-yabJ-spoVG) or the empty control plasmid pGC2. Bacteria were stained with DAPI, marked with CP-5-specific monoclonal antibodies, and stained with Cy3-conjugated anti-mouse antibodies (CY-3).