TABLE 2.
Primers used in this study
| Primer | Sequence (5′-3′)a | Locationb or reference |
|---|---|---|
| arlRprobe+ | CAATGTGGACACAGAGTATG | 1465970-1465989 |
| arlRprobe− | CAGTTCGTGGCGTTGGG | 1465422-1465438 |
| arlSprobe+ | CAGCAGTATTAGAAGAATCG | 1464595-1464614 |
| arlSprobe− | GAGTCCATTACCGCCTTGAC | 1464154-1464173 |
| Asp23probe+ | ATGACTGTAGATAACAATAAAGC | 11 |
| Asp23probe− | TTGTAAACCTTGTCTTTCTTGG | 11 |
| capAp-F | cgcaagcttCAAACATCATATGATTATAAGC | 153034-153055 |
| capAp-R | gcgccatggTTTACCTCCCTTAAAAATT | 153384-153402 |
| oSTM01 | taggtaccTCAAAAGAAGTTAAACAAAG | 548665-548684 |
| oSTM02 | cggatccATATTAATCGAAAATTATAATTCC | 549175-549198 |
| oSTM03 | cgcctgcagATTATGATGATATGAAAATTATTG | 549706-549729 |
| oSTM04 | gcgaagcttGACCAATAACAACATCTTCGCC | 550760-550781 |
| oSTM20 | GAAAATCATTAACACAACAAG | 548806-548826 |
| oSTM21 | CTTAATTTTACTTACTAATTC | 549155-549175 |
| oSTM28 | cgatggatccGTGTTATGAATTTAATGAATGAG | 548603-548625 |
| oSTM29 | gcgtcgacTTATTGCAAATGTATTACATCGC | 549574-549596 |
| oSTM30 | CTAAATAAAACAGAGAGATATATACTATAGG | 549213-549243 |
| oSTM31 | gcgggtaccGTGTTATGAATTTAATGAATGAG | 548603-548625 |
| oSTM32 | gcgggatccGAAGCTTGATTAAACATATTAATCG | 549189-549213 |
| oSTM33 | gcgggatccTTATTGCAAATGTATTACATCGC | 549574-549596 |
| oSTM34 | ggattttcatatgACTAAAACTCCTTTTATGAAAAC | 548778-548800 |
| oSTM61 | cgcggatccCAAACATCATATGATTATAAGC | 153034-153055 |
| oSTM62 | gcgctcgagTTTACCTCCCTTAAAAATT | 153384-153402 |
| oSTM63 | cgcggatccGCAGTAAACCTAAAGTGTCG | 1466817-1466836 |
| oSTM64 | gcgctcgagTTGTACACCTCATATTACGAC | 1466067-1466087 |
| oSTM71 | gtgcatatgAAAGTGACAGATGTAAGACTTAG | 549259-549281 |
a
Lowercase letters represent nucleotide additions.
b
Based on the sequence of strain COL (RefSeq accession no. NC_002951).