FIG. 6.

Effect of the bclA mutation on uptake of spores by macrophages. RAW264.7 cells were infected with spores at an MOI of 1 to 3 for one cell. (A) Data depict viable counts. The error bars represent standard errors of the means. (B) Microscopic counts of fluorescently labeled samples. (C) Microscopic counts of samples stained with the malachite green spore stain. The phagocytic index is the mean number of spores phagocytosed per macrophage multiplied by 100. Similar results were obtained in at least two additional experiments.