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. 2007 Jun 25;75(9):4597–4607. doi: 10.1128/IAI.00630-07

FIG. 1.

FIG. 1.

Agarose plug chemotaxis. Attraction or repulsion of E. coli O157:H7 to different chemical agents was determined using a modified agarose plug assay. Fluorescence images from bacteria exposed to 500 μM EPI (A), 500 μM NE (B), 2% Casamino Acids (C), 5,000 μM indole (D), 1 × 10−4 M glycerol (E), and 1× M9 salts solution (F) are shown. Green cells are E. coli O157:H7 expressing GFP, and red cells are kanamycin-killed E. coli TG1 expressing RFP. Fluorescence images were obtained on a Zeiss Axiovert 200 microscope after 30 min using a 10× objective. Data shown are representative images from three independent experiments. Arrows indicate chemoattractant ring (A), clearance zone (C), and chemorepellant band (D).