FIG. 1.
CHO cell contact-mediated induction of T3SS gene expression requires a functional secretion/translocation channel. (A) P. aeruginosa strains carrying pJNE05 encoding the exoS-gfp transcriptional fusion were grown under Ca2+-replete (−EGTA) or -limiting (+EGTA) conditions, and GFP fluorescence was examined by flow cytometry. wt, wild type. (B) P. aeruginosa strains carrying pJNE05 were cultured either in the presence (+ CHO cells) or in the absence (− CHO cells) of CHO cells as indicated, and then GFP fluorescence was examined by flow cytometry. The curves plot the relative number of bacterial cells (y axis) at each relative level of GFP fluorescence (x axis).