FIG. 2.

Activation of iNOS and IRF-1 expression and phosphorylation of Y701-STAT-1 in mouse macrophages infected with the LPS mutant. Mouse macrophages were infected with the LPS mutant or the wild-type B. pseudomallei at an MOI of 2:1 for 1 h. After 4, 6, and 8 h of infection, the levels of iNOS, IRF-1, and actin expression (A) and of phosphorylated Y701-STAT-1 and STAT-1 (B) were determined by immunoblotting. The data showed all of these components to be upregulated in the macrophages infected with the mutant compared with those infected with its wild-type counterpart.