FIG. 5.

C. burnetii infection does not induce mass degradation of proapoptotic Bcl-2 family proteins. THP-1 cells were infected with the C. burnetii Nine Mile phase II strain for 48 h. Infected and uninfected cell cultures were then treated with staurosporine for 2 h. Cells were lysed, and equal amounts of protein were subjected to immunoblot analysis using Bad, Bak, Bax, Bik, Bim, or Bmf primary antibodies. Similar levels of these proteins were observed between infected and uninfected cells treated with staurosporine. The GAPDH immunoblot is included as a loading control.