FIG. 1.

CD4⁺ CD25^high FoxP3⁺ T cells are induced from naïve T cells after incubation with H. pylori-exposed GEC. Shown are (A) naïve T cells incubated with HS-738 cells for forward and side scatter gates, (B) CD4 and CD25 staining of freshly isolated naïve T cells, (C) FITC and PE isotype controls, (D) naïve T cells incubated with HS-738 cells plus isoptype control for 7 days and stained with anti-CD25 FITC and FoxP3 PE monoclonal antibodies, and (E) naïve T cells incubated with H. pylori-exposed HS-738 cells plus isotype control for 7 days and stained with anti-CD25 FITC and FoxP3 PE monoclonal antibodies. Representative results of five experiments are shown.