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Secreted hemolytic activity of the traP mutant. S. aureus strains were streaked onto TSA sheep blood agar (Remel) and incubated for either 24 h (8325-4 lineage strains) (A) or 48 h (Newman lineage strains) (B). Tight zones of clearing around the immediate periphery of strains indicate secreted α-hemolysin, while the more diffuse zones around the strains correspond to secreted β-hemolysin.
