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. 2007 Jun 4;75(9):4519–4527. doi: 10.1128/IAI.00491-07

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Copyright © 2007, American Society for Microbiology

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FIG. 3. — Secreted and surface-associated protein profiles of the traP mutant. (A) Secreted protein profiles of 8325-4 (lane 1), 8325-4 traP::kan (lane 2), and 8325-4 agr::tet (lane 3). Protein samples are equivalent to 1.0 OD₆₀₀ unit of original culture. (B) Secreted protein profiles of Newman (lane 1), Newman traP::kan (lane 2), and Newman agr::tet (lane 3). Protein samples are equivalent to 2.0 OD₆₀₀ units of original culture. (C) Surface-associated protein profiles of 8325-4 (lane 1), 8325-4 traP::kan (lane 2), and 8325-4 agr::tet (lane 3). Protein samples are equivalent to 150 OD₆₀₀ units of original culture. (D) Surface-associated protein profiles of Newman (lane 1), Newman traP::kan (lane 2), and Newman agr::tet (lane 3). Protein samples are equivalent to 75 OD₆₀₀ units of original culture. The arrow denotes the 55-kDa band corresponding to surface protein A, which was subjected to densitometric analysis.