TABLE 1.
Oligonucleotide primers used in this study
| Primer | Nucleotide sequencea |
|---|---|
| P2 | 5′-TACCTACAACCTCAAGCT-3′ |
| STM-R2 | 5′-CAAACCGTTATTCATTCG-3′ |
| rpeA-F | 5′-CTTACACGTGGCACTAG-3′ |
| rpeA-R | 5′-CAGTGATCGCTGAAGGAG-3′ |
| ral-F | 5′-ATGGATAAGCGTACGCC-3′ |
| ral-R | 5′-AATACTAACTGGTAGGG-3′ |
| Tn5F | 5′-CAGGCATGCAAGCTTCG-3′ |
| Tn5R | 5′-GGTACCGAGCTCGAATT-3′ |
| prpeA-F | 5′-CGGGATCCCGATCTCGAACAACATTTACACG-3′ |
| prpeA-R | 5′-CGGGATCCCGTTACTTGTCGTCATCGTCTTTGTAGTCGAACTCATATTTCAAACCAAG-3′ |
| pKD4rpeA-F | 5′-ATGAACAAGATTTTTTCACTTAAATTTAGCACCGCTTCTGGTGGTTTAATTGCATGTGTAGGCTGGAGCTGCTTCG-3′ |
| pKD4rpeA-R | 5′-TCAGAACTCATATTTCAAACCAAGAATTCCAACAGTATCGGTTTTGAAGTCACCACCATATGAATATCCTCCTTA-3′ |
| ralI-F | 5′-CTGGTACTGGGAAATTAC-3′ |
| rpeA-RT | 5′-GAAGCAATATGGCGTCG-3′ |
| ralG-F | 5′-CAAATTCAGTTATTGGTAC-3′ |
| ralH-R | 5′-CCATTTTGGAGCCTCATC-3′ |
a
Engineered restriction sites are underlined, and pKD4 sequences are italicized.