TABLE 2.
Distribution of stx types, eae subtypes, and nleA variants among 170 pathogenic E. coli isolates and restriction fragment lengths from Southern blot hybridizations with an nleA4795 probe
| Serotype (no of isolates) | Host | Diseasea (no. of cases) | stx type | eae type | nleA variant(s) | Hybridization product size(s) in kb (no. of products) |
|---|---|---|---|---|---|---|
| O15:H− (1) | Rabbit | D | β | Z6024 | ||
| O26:H11 (1) | Human | NK | 1 | β | Z6024,bnleA8-1 | 8.6, 11.8 |
| O26:H11 (3) | Human | HUS | 2 | β | Z6024,bnleA8-1 | 12, 24 (2) |
| O26:H11 (2) | Human | HUS (1), NK (1) | 2 | β | nleA8-1 | 25 |
| O26:H11 (2) | Human | HUS (1), D (1) | 1 | β | Z6024,bnleA8-1 | 12, 24 |
| O26:H− (1) | Human | NK | 1/2 | β | Z6024,bnleA8-1 | 8.6, 11.8 |
| O26:H− (2) | Human | D (1), AS (1) | 1 | β | Z6024,bnleA8-1 | 12, 24 |
| O26:H− (2) | Human | NK (1), AS (1) | 2 | β | Z6024,bnleA8-1 | 12, 24 |
| O26:H− (5) | Human | HUS (4), D (1) | 2 | β | Z6024,bnleA8-1 | 12, 24 |
| O49:H2 (3) | Human | D | β | espI-like gene | 20 (2) | |
| O49:H2 (1) | Human | D | β | No signal | ||
| O49:H2 (1) | Rabbit | NK | β | espI-like gene | 4.3 | |
| O49:H10 (1) | Dog | NK | κ | Z6024 | 9 | |
| O49:H18 (1) | Pig | NK | κ | nleA2 | 16 | |
| O49:H35 (1) | Cattle | NK | κ | nleA2 | 17 | |
| O49:NM (1) | Human | HC | κ | nleA10 | 4.8 | |
| O49:NM (1) | Cattle | NK | κ | No signal | ||
| O49:NM (1) | Dog | D | No signal | |||
| O49:NM (3) | Pig | NK | β | nleA8-1, nleA8-1 | 4.5, 23 | |
| O84:H2 (1) | Cattle | NK | 1 | ζ | nleA4 | 24 |
| O84:H2 (1) | Human | D | 1 | ζ | nleA9, nleA8-2c | 7, 30 |
| O84:H2 (1) | Human | NK | 1 | ζ | nleA9, nleA8-2c | 30 |
| O84:H2 (1) | Human | D | 1 | ζ | nleA7, nleA8-2c | 6.8, 30 |
| O84:H4 (1) | Human | D | 1 | ζ | nleA4795 | 11 |
| O84:H28 (2) | Human | NK | 1 | ζ | nleA4795 | 11 (1) |
| O84:H− (1) | Human | D | 1 | ζ | nleA9, nleA8-2c | 30 |
| O84:H− (1) | Human | D | 1 | ζ | nleA7, nleA8-2c | 6.8, 30 |
| O84:H− (2) | Cattle/sheep | NK | 1 | ζ | nleA7, nleA8-2c | 6.8, 30 |
| O84:H− (1) | Pig | NK | θ | nleA8-2, nleA8-1b | 13, 25 | |
| O84:nt (1) | Human | D | 1 | ζ | nleA9 | 30 |
| O84:nt (2) | Human | D (1), NK (1) | 1 | ζ | nleA7, nleA8-2c | 6.8, 30 |
| O84:nt (1) | Human | D | 1 | ζ | nleA9, nleA8-2c | 7, 30 |
| O103:H2 (2) | Rabbit/chicken | D | β | espI-like | 13 | |
| O103:H2 (1) | Meat | 1 | ɛ | espI-like | 24.5 | |
| O103:H2 (1) | Human | SIDS | ɛ | espI-like | 24.5 | |
| O103:H2 (1) | Human | D | β | espI-like, nleA11 | 7, 24.5 | |
| O103:H2 (3) | Human | HUS | 2 | ɛ | espI-like | 24.5 |
| O103:H2 (3) | Human | NK (2), AS (1) | 1 | ɛ | espI-like | 24.5 (3) |
| O103:H2 (1) | Human | NK | 1 | espI-like | 24.5 | |
| O103:H2 (4) | Human | HUS (3), UTI (1) | 1 | ɛ | espI-like | 24.5 (3) |
| O103:H7 (1) | Dog | HC | No signal | |||
| O103:H11 (1) | Human | NK | 1 | β | nleA8-1 | 25 |
| O103:H18 (1) | Human | D | 1 | ɛ | espI-like | 24.5 |
| O103:H− (2) | Human | HUS (1), D (1) | 1 | ɛ | espI-like | 24.5 (1) |
| O103:H− (1) | Human | HUS | 2 | ɛ | espI-like | 24.5 |
| O111:H2 (1) | Human | NK | β | espI-like | 25 | |
| O111:H2 (1) | Human | NK | θ | Z6024 | 11.2 | |
| O111:H2 (2) | Human | D | 1 | θ | nleA8-1 | 27 (1) |
| O111:H8 (1) | Human | HUS | 1 | θ | nleA8-1 | 27 |
| O111:H− (1) | Human | NK | β | espI-like | ||
| O111:H− (6) | Human | HUS (2), D (4) | 1 | θ | nleA8-1 | 27 (4) |
| O111:H− (7) | Human | HUS | 1/2 | θ | nleA8-1 | 27 (2) |
| O111:H− (1) | Human | HUS | 1/2 | nleA8-1 | 27 | |
| O118:H5 (1) | Human | NK | κ | Z6024 | ||
| O125:H− (1) | Human | D | η | nleA8-2 | 12 | |
| O127:H6 (1) | Human | NK | α | No signal | ||
| O128:H2 (3) | Human | D (2), AS (1) | 1/2 | No signal (3) | ||
| O128:H2 (7) | Human | D | β | espI-like | 20 (3) | |
| O128:H2 (2) | Rabbit/chicken | D (1), AS (1) | β | espI-like | 20 | |
| O128:H2 (1) | Sheep | AS | 1/2 | No signal | ||
| O128:H2 (2) | Pigeon | AS | 2f | β | espI-like | 10 (1) |
| O128:B12 (1) | Human | D | 2f | β | espI-like | 10 |
| O128:H− (1) | Human | D | 1 | No signal | ||
| O128:H− (2) | Human | D (1), AS (1) | 1/2 | No signal (2) | ||
| O145:H4 (1) | Human | NK | ι | No signal | ||
| O145:H28 (1) | Human | HUS | 2 | γ | nleA3 | 5 |
| O145:H28 (2) | Human | D | 1 | γ | nleA5 | 5.5 |
| O145:H28 (1) | Human | HUS | 2 | γ | nleA6-1 | 9.8 |
| O145:H28 (1) | Human | D | No signal | |||
| O145:H28 (1) | Pig | AS | γ | nleA11 | 19 | |
| O145:H34 (1) | Human | D | ι | No signal | ||
| O145:NM (6) | Human | HUS (5), D (1) | 2 | γ | nleA2 | 30 (2) |
| O145:NM (2) | Human | HUS (1), D (1) | 1 | γ | nleA2 | 30 (2) |
| O145:NM (1) | Cattle | AS | 1 | γ | nleA2 | 30 |
| O145:NM (1) | Pig | NK | γ | nleA2 | ||
| O145:NM (1) | Human | HUS | 2 | β | Z6024 | 31 |
| O145:NM (1) | Human | HUS | 1/2 | γ | nleA2 | 30 |
| O156:H1 (1) | Human | AS | ζ | 1 | 6 | |
| O156:H8 (4) | Human | NK (3), AS (1) | θ | No signal (4) | ||
| O156:H8 (2) | Pig | NK (1), D (1) | θ | Z6024 | 9 (1) | |
| O156:H21 (1) | Human | D | 1/2 | No signal | ||
| O156:H21 (1) | Cattle | NK | 1 | ζ | nleA3, nleA8-2c | 4.8, 6.2 |
| O156:H25 (1) | Human | AS | 1 | ζ | nleA3, nleA8-2c | 4.8, 6.2 |
| O156:H25 (1) | Human | NK | θ | nleA6-2, nleA8-2c | 6.2, 9.8 | |
| O156:H25 (1) | Human | NK | ζ | nleA6-1, nleA8-2c | 6.2, 9.8 | |
| O156:H25 (1) | Cattle | NK | ζ | nleA3, nleA8-2c | 5.5, 6.2 | |
| O156:H25 (4) | Sheep | NK | 1 | ζ | nleA6-1, nleA8-2c | 6.2, 11.5 (4) |
| O157:H7 (1) | Human | D | γ | Z6024 | 10 | |
| O157:H7 (1) | Ground beef | 1/2 | γ | Z6024 | 12 | |
| O157:H7 (4) | Human | HUS (3), AS (1) | 1/2 | γ | Z6024 | 12 (1) |
| O157:H7 (4) | Human | HUS (2), D (2) | 2 | γ | Z6024 | 12 (2) |
| O157:H− (1) | Human | NK | γ | Z6024 | ||
| O157:H− (2) | Human | D (1), AS (1) | 1/2 | γ | Z6024 | 12 (2) |
| O157:H− (1) | Human | E | 1/2 | γ | Z6024 | |
| O157:H− (2) | Human | HUS | 2 | γ | Z6024 | 9 (1), 12 (1) |
| O157:H− (4) | Human | HUS (3), AS (1) | 2 | γ | Z6024 |
a
NK, not known; D, diarrhea; AS, asymptomatic; HC, hemorrhagic colitis; SIDS, sudden infant death syndrome; UTI, urinary tract infection; E, enteritis.
b
Variant gene with a 1-bp deletion of either the Z6024 or nleA8-1 sequence.
c
The 5′ end of nleA8-2 was missing from this gene.