FIG. 2.

Detection of target viruses by the RMA is highly sensitive and specific. Each well, containing 20 copies of cDNA of the target genomic regions (Table 2) of the indicated target virus, panel A (RSV A and B; PIV1, -2, -3, -4a, and -4b; InfV A and B; MPV; and HRV1A) and panel B (Ad3 [group B], Ad1 [group C], Ad4 [group E], CoV 229E, CoV NL63, CoV OC43, EV68, EV69, EV70, EV71, CA22 [coxsackievirus A22], and CB1 [coxsackievirus B1]), was analyzed with the RMA. Human genomic DNA (60,000 copies per well) was used as the negative control (NC). Each bar represents the average MFI of triplicate samples. The error bars indicate standard deviations.