TABLE 1.
Real-time PCR primers
| PCR primer | Target | Sequence | Nucleotide positionsa |
|---|---|---|---|
| HSV 1:3 | Glycoprotein D | 5′-CCATACCGACCACACCGACGA-3′ | 55-74 |
| HSV 1:2 | Glycoprotein D | 5′-CATACCGGAACGCACCACAC-3′ | 243-224 |
| HSV 2:1 | Glycoprotein G | 5′-TCAGCCCATCCTCCTTCGGCAGTA-3′ | 138240-138262 |
| HSV 2:4 | Glycoprotein G | 5′-CGCGCGGTCCCAGATCGGCA-3′ | 138398-138380 |
| VZV fw | ORF 29 | 5′-TGTCCTAGAGGAGGTTTTATCTG-3′ | 53941-53963 |
| VZV rev | ORF 29 | 5′-CATCGTCTGTAAGACTTAACCAG-3′ | 54142-54120 |
a
Derived from GenBank accession numbers J02217 for HSV-1, Z86099 for HSV-2, and X04370 for varicella-zoster virus.